Nano Spectrophotometer BEY1R1 (BSNA-206)

Biophotometer
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  • 1 Year Warranty
  • US & European Standards



Nano Spectrophotometer Xenon Lamp CCD (2048 Pixels)

Specifications

ModelBEY1R1
Generation Specification
- Light SourceXenon Lamp
- DetectorCCD (2048 Pixels)
- Wavelength Range190-1100 nm
- Wavelength Accuracy±1 nm
- Spectral Resolution0.3 nm
- Dimension230x290x220 mm
- Weight3 kg
- Operating Voltage12 V DC
- Power Consumption18 W
Nano Volume Specification
- Absorbance Precision1%@100 ng/μL
- Absorbance Range0-200 Abs (10 nm equivalent)
- Detection Limit2 ng/μL (dsDNA)
- Max. Concentration15000 ng/μL (dsDNA)
- Measurement Time5 s
- Min. Sample Volume1 μL
- Path Length0.01-1.2 mm (auto-ranging)
Cuvette Specification
- Beam Height8.5mm
- Absorbance Range0.002-2.0Abs
- Measurement Time3s
- Path Length10mm

Features

• High-quality flash Xenon lamp, longer service life.
• High resolution CCD detector, fast measurement (5s), high repeatability and accuracy.
• 7 inches LCD color touch screen, stand-alone system, no need for a PC.
• User-friendly operating system, module UI design, simple and clear.
• No need to dilute the sample, 1uL to 2uL is enough.
• Multi-functions: Nucleic acid , Protein, Kinetics, Spectrum scanning, End-point and OD600 measurement.
• Available to upgrade software by USB, low maintenance cost.
• Storage by USB, simple to retrieve test data.
• Two USB ports for data transmission and outer tools like mouse and keyboard.
• Smart and compact design, light and easy to move.


• High-quality flash Xenon lamp, longer service life. • High resolution CCD detector, fast measurement (5s), high

Nano Spectrophotometer Xenon Lamp CCD (2048 Pixels)

Nano Spectrophotometer Xenon Lamp CCD (2048 Pixels)

Nano Spectrophotometer Xenon Lamp CCD (2048 Pixels)

Nano Spectrophotometer Xenon Lamp CCD (2048 Pixels)

Nano Spectrophotometer Xenon Lamp CCD (2048 Pixels)

Photos

BSNA-206

Operating Manual

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1. Introduction
    1.1 Measurement Principle
    1.2 Performance and Features
    1.3 Applications
    1.4 Packing List
    1.5 Product Design
    1.6 Technical Specifications
    1.7 Maintenance
2. Basic Use
    2.1 Pedestal
    2.2 Cuvette
    2.3 Blank Calibration
    2.4 Keyboard
3. User Interface
    3.1 Login
    3.2 Main interface
    3.3 Submenu
    3.4 Nucleic Acids Measurement
    3.5 Protein Measurement
    3.6 End Point and OD600 Measurement
    3.7 Kinetic Measurement
    3.8 Spectrum Measurement
    3.9 Search
    3.10 Account Setting
    3.11 Power Panel
    3.12 Setup

1. Introduction

1.1 Measurement Principle

The measurement principle of bio-photometer is based on the Lambert-Beer law. When the beam of collimated monochromatic light passes through a certain uniform colored solution, the absorbance of the solution is directly proportional to the concentration of the solution and the optical path. And it supplies basis for the quantitative analysis. The Lambert-Beer law is described as following formula:

A=kxbxC

A - Absorbance of the analyte.

k - The absorption coefficient.

b - The path length in cm.

c - The analyte concentration.

1.2 Performance and Features

The performance and features of BSNA-206 Bio-photometer are as following:

• Imported long service life UV-Visible light source (Xenon Flash Lamp), without pre-warming.

• 7 inches LCD touch screen, enables the measurement, data storage and transmission without computer.

• Friendly operation system and graphic operation interface, enables easy operation.

• High resolution CCD detector, enables fast detection in five seconds, with good repeatability and high accuracy.

• Micro volume detection with less sample consumption as low as 1L, enables high concentration sample measurement without dilution.

• Powerful measurement functions such as nucleic acids measurement, protein measurement, kinetic measurement, spectrum measurement, end point measurement and OD600 measurement are available.

• Two USB ports, enables data transmission by USB Flash Drive, instrument operation with mouse and keyboard.

• The measurement results and graphs are clearly displayed, and the graph can be screen captured. The data can be backed up and saved to USB Flash Drive, and the storage path is easy for search.

• Convenient data search, and history data can be reviewed according to title, time, or measurement item, enables data easily exported and backed up.

• High performance without maintenance, and system upgrade is available with USB Flash Drive.

CUVETTE & MICROVOLUME MEASUREMENT

Samples in universal cuvette or microvolume can be measured.

Tags:, Description:, Attributes-End, The measurement principle of bio-photometer is based on the Lambert-Beer law. When the

EASY UPGRADES

The system can be upgraded with ease via USB Flash Drive.

MINIMUM MAINTENANCE

No recalibration or internal maintenance is needed.

1.3 Applications

BSNA-206 Bio-photometer is designed for both cuvette and microvolume samples. The valid wavelength range is 190nm-1100nm. It's applied to fast detection of samples such as DNA, RNA and proteins. Universal sample measurement with cuvette is also available. With high accuracy and superb repeatability, it has special advantages in the fields of biochemistry analysis, teaching and scientific research.

1.4 Packing List

NO.

ITEM

UNIT

QTY.

NOTE

1

BSNA-206 Bio-photometer

set

1

2

Pedestal Cover

pc

1

Fig. 1-1

3

Blocking Stick

pc

1

Fig. 1-1

4

Cuvette

pc

1

Fig. 1-1

5

Power Cord

pc

1

6

Power Adaptor

pc

1

7

User's Manual

pc

1

Table 1

Table 1, Figure 1, The main structure of BSNA-206 Bio-photometer is shown in Figure, Figure 2, The right side and back side

Figure 1

1.5 Product Design

The main structure of BSNA-206 Bio-photometer is shown in Figure

Table 1, Figure 1, The main structure of BSNA-206 Bio-photometer is shown in Figure, Figure 2, The right side and back side

Figure 2

The right side and back side of BSNA-206 Bio-photometer are respectively shown in Fig. 3 and Fig. 4:

Table 1, Figure 1, The main structure of BSNA-206 Bio-photometer is shown in Figure, Figure 2, The right side and back side

Figure 3

Table 1, Figure 1, The main structure of BSNA-206 Bio-photometer is shown in Figure, Figure 2, The right side and back side

Figure 4

1.6 Technical Specifications

General Specification

Light Source

Xenon Flash Lamp

Detector

CCD (2048 Pixels)

Wavelength Accuracy

±1 nm

Wavelength Range

190 nm - 1100 nm

Spectral Resolution

0.3 nm

Dimensions

230 mm x 290 mm x 220 mm (W x D x H)

Weight

3 kg

Operator Voltage

12 V DC

Power Consumption

18 W

Warranty

1 Year

LCD Touch Panel

7.0 inch

Table 2

Micro Volume Specification

Absorbance Precision

1% (@100 ng/L)

Absorbance Range

0 -300 Abs. (equivalent to 10 mm path length)

Detection Limit

2 ng/L (dsDNA)

Maximum Concentration

15000 ng/L (dsDNA)

Measurement Time

5 s

Minimum Sample Size

1 L

Path Length

0.01 - 1.2 mm (Auto-ranging)

Table 3

Cuvette Specification

Beam Height

8.5 mm

Absorbance Range

0.002 - 2.0 Abs.

Measurement Time

3 s

Table 4

1.7 Maintenance

• Due to fixed modules and immovable parts, internal maintenance is not needed.

• Clean the exterior with a clean and dry wipe when there is a spill or contamination.

• DO NOT try to disassemble the instrument in any situation.

• Use an electric supply that conforms to industry standards.

• Place the instrument in a clean environment and away from other devices that vibrate (e.g. centrifuges).

• Test the general functions of the device - check buttons and the power switch.

• Confirm that the mechanical components are in good condition.

• Make sure accessories, cables, and positions are clean and intact.

• Calibration and update from the manufacturer is possible.

• It is recommended that user keep the instrument plugged in even when not in use.

2. Basic Use

The basic operation process of BSNA-206 Bio-photometer is described in Figure

Table 1, Figure 1, The main structure of BSNA-206 Bio-photometer is shown in Figure, Figure 2, The right side and back side

Figure 5

Sample insertion of the two detection modes (Cuvette Mode and Micro Volume Mode) is shown in Fig.6

Table 1, Figure 1, The main structure of BSNA-206 Bio-photometer is shown in Figure, Figure 2, The right side and back side

Figure 6

2.1 Pedestal

Micro volume sample measurement includes the use of the Pedestal. The Pedestal has upper and lower pedestals. Targeted micro volume sample should be gently pipetted on the center top of the lower pedestal, and the Blocking Stick should be inserted during the micro volume measurement. The sample should be wiped off from both lower and upper pedestals with a dry and lint-free laboratory wipe after the measurement.

Procedure for Pedestal Cleaning

The brief process diagram is shown in Fig.7

Table 1, Figure 1, The main structure of BSNA-206 Bio-photometer is shown in Figure, Figure 2, The right side and back side

Figure 7

1. Open the upper cover of the instrument.

2. For standard cleaning, pipette 5 μl distilled water onto the lower pedestal.

• Use 0.5M HCl instead of distilled water to clean dried proteins from the pedestal.

• For decontamination, use a solution of 0.5% sodium hypochlorite (1:10 dilution) or other sanitizing solution instead. Then, repeat the process with distilled water.

3. Close the upper cover of the instrument and wait 3 minutes.

4. Open the upper cover of the instrument and wipe both the upper and lower pedestals with a dry and lint-free laboratory wipe.


Note: Do not use isopropyl alcohol or detergents for pedestal cleaning.

Procedure for Pedestal Reconditioning

1. Open the upper cover of the instrument.

2. Apply a tiny amount of the pedestal reconditioning compound to the surfaces of the upper and lower pedestals. Apply the compound in a very thin layer, making sure that it stays as even as possible.

3. Wait at least 30 seconds for the compound to dry completely.

4. Wipe away the compound with a clean, dry laboratory wipe. Make sure to wipe away the compound completely by rubbing the pedestal surfaces very thoroughly.

2.2 Cuvette

Cuvette measurement includes the use of cuvette. The target sample should be prepared in a universal cuvette, and the cuvette should be gently inserted in the cuvette port for the measurement. The lower pedestal should be covered by the Pedestal Cover during the cuvette measurement. The direction of the light pathway during the measurement is indicated on the upper side of the port. The minimum volume of the sample is 1 ml.

Note: Do not use isopropyl alcohol or detergents for pedestal cleaning., Procedure for Pedestal Reconditioning, 1. Open the

Figure 8


Note: Make sure the V shape side is facing the same direction as the arrow on top.


Procedure for Cuvette Cleaning

1. Open the upper cover of the instrument.

2. Use a dry and lint-free laboratory wipe to wipe away any spills inside or around the cuvette port.

3. If there is excess lint or dust in or around the cuvette port, use canned air to clean out the cuvette port.

4. Ensure cuvettes are clean and free of scratches before use.

Note: Do not squirt water or any other liquid into the cuvette port for cleaning.

2.3 Blank Calibration

Procedure for Blank Calibration of Micro Volume Measurement

1. Open the upper cover of the instrument.

2. Make sure to insert the Blocking Stick in the cuvette port.

3. Gently pipette the blank sample onto the lower pedestal, close the upper cover.

4. Touch the button " Procedure for Cuvette Cleaning, 1. Open the upper cover of the instrument., 2. Use a dry and lint-free laboratory wipe to " on the measurement interface to perform blank calibration.

5. After completing the blank calibration, open the upper cover and wipe off the blank sample.


Procedure for Blank Calibration of Cuvette Measurement

1. Prepare the blank sample in a universal cuvette. The minimum volume of the sample is 1 ml.

2. Make sure to cover the lower pedestal with the Pedestal Cover.

3. Insert the cuvette in the cuvette port.

4. Touch the button "" to perform blank calibration.

5. After completing the blank calibration, open the upper cover and remove the cuvette.

Procedure for Blank Calibration of Cuvette Measurement

1. Prepare the blank sample in a universal cuvette. The minimum volume of the sample is 1 ml.

2. Make sure to cover the lower pedestal with the Pedestal Cover.

3. Insert the cuvette in the cuvette port.

4. Touch the button " Procedure for Cuvette Cleaning, 1. Open the upper cover of the instrument., 2. Use a dry and lint-free laboratory wipe to " to perform blank calibration.

5. After completing the blank calibration, open the upper cover and remove the cuvette.

2.4 Keyboard

There is an internal keyboard functionality for the touch screen. However, users may make use of their own keyboard and mouse by connecting them through the USB ports located on the right side of the instrument.

The keyboard (Fig. 9) will automatically pop up when any letter box (e.g. title) is touched. When the button " Procedure for Blank Calibration of Cuvette Measurement, 1. Prepare the blank sample in a universal cuvette. The minimum " that located at the bottom left corner of the keyboard is touched, it will convert to the number and special letter pad (Fig. 10). User can minimize the keyboard by touching the button " Procedure for Blank Calibration of Cuvette Measurement, 1. Prepare the blank sample in a universal cuvette. The minimum " that located at the upper right corner of the keyboard. User can also freely move the keyboard, by touching the button " Procedure for Blank Calibration of Cuvette Measurement, 1. Prepare the blank sample in a universal cuvette. The minimum " next to " Procedure for Blank Calibration of Cuvette Measurement, 1. Prepare the blank sample in a universal cuvette. The minimum ".

Procedure for Blank Calibration of Cuvette Measurement, 1. Prepare the blank sample in a universal cuvette. The minimum

Figure 9

Procedure for Blank Calibration of Cuvette Measurement, 1. Prepare the blank sample in a universal cuvette. The minimum

Figure 10

3. User Interface

3.1 Login

When the instrument is turned on, the Login interface will appear as above (Fig. 3-1). Users can enter their ID and Password, and touch the button " Procedure for Blank Calibration of Cuvette Measurement, 1. Prepare the blank sample in a universal cuvette. The minimum " to login, or can login as a guest by touching the button " Procedure for Blank Calibration of Cuvette Measurement, 1. Prepare the blank sample in a universal cuvette. The minimum " without entering the User ID and Password for quick measurement.

Procedure for Blank Calibration of Cuvette Measurement, 1. Prepare the blank sample in a universal cuvette. The minimum

Figure 11

User ID

User ID of the account.

Password

Password of the account.

Procedure for Blank Calibration of Cuvette Measurement, 1. Prepare the blank sample in a universal cuvette. The minimum

Login as an account with specified ID.

Procedure for Blank Calibration of Cuvette Measurement, 1. Prepare the blank sample in a universal cuvette. The minimum

Login as a guest.

Table 5

3.2 Main interface

When user login, the main operation interface will appear as above (Fig. 3-2). User can access to any measurement interface from the main interface.

Table 5, When user login, the main operation interface will appear as above (Fig. 3-2). User can access to any measurement

Figure 12

BSNA-206

To enter Nucleic Acids measurement interface. (pg. 11)

BSNA-206

To open Protein menu. (pg. 12)

BSNA-206

To enter OD600 measurement interface. (pg. 16)

BSNA-206

To enter End Point measurement interface. (pg. 16)

BSNA-206

To enter Kinetic measurement interface. (pg. 17)

BSNA-206

To enter Spectrum measurement interface. (pg. 17)

BSNA-206

To open Account Settings panel. (pg. 21)

BSNA-206

To open Search panel. (pg. 18)

BSNA-206

To open Setup panel. (pg. 23)

BSNA-206

To open Power Option panel. (pg. 22)

Table 6

3.3 Submenu

There are submenus located at the top of every measurement interface

Table 6, There are submenus located at the top of every measurement interface BSNA-206

BSNA-206

Return to the Main interface.

BSNA-206

To open Search panel.

BSNA-206

To capture current screen and save it in the USB Flash Drive connected. (Refer to pg. 10)

BSNA-206

To perform blank calibration.

BSNA-206

To perform measurement according to the settings.

Table 7

The button " BSNA-206 " is only in Nucleic Acids, Kinetic, Protein, and Spectrum interfaces.

User may capture a screenshot of the screen by touching the button " BSNA-206 " in Nucleic Acids, Kinetic, Protein, Spectrum measurement interfaces, and save it as image files (.JPG) in a USB Flash Drive. The screenshot image files will be saved in the "Capture" folder under the "Biolab" folder.


Note: The USB Flash Drive must be plugged in one of the USB ports on the right side of the instrument before the capture operation.

3.4 Nucleic Acids Measurement

In Nucleic Acids measurement interface (Fig. 3-4), user can measure nucleic acids samples. There are various options for Nucleic Acids measurement: dsDNA, ssDNA, RNA, and Oligo.

Note: The USB Flash Drive must be plugged in one of the USB ports on the right side of the instrument before the capture

Figure 13

BSNA-206

To measure double-stranded DNA samples.

BSNA-206

To measure single-stranded DNA samples.

BSNA-206

To measure RNA samples.

BSNA-206

To measure Oligonucleotide samples.

BSNA-206

Overlay Button: To enter Overlay interface.

Title

Title of the measurement.

Conc.

Shows the concentration of the sample.

260nm (10mm path)

Shows the Abs. value at 260nm.

280nm (10mm path)

Shows the Abs. value at 280nm.

230nm (10mm path)

Shows the Abs. value at 230nm.

260/280 ratio

Shows the calculated ratio of Absorbance values at the wavelength of 260 and 280nm.

260/230 ratio

Shows the calculated ratio of Absorbance values at the wavelength of 260 and 230nm.

Table 8

Procedure for Nucleic Acids Measurement

1. Enter Nucleic Acids measurement interface by touching the button " BSNA-206 " in the Main interface.

2. Touch and select the measurement item among dsDNA, ssDNA, RNA, and Oligo.

3. Input the title of the measurement.

4. Open the upper cover of the instrument.

5. Perform the blank calibration. (Refer to pg. 7)

6. Pipette microvolume of the target sample onto the lower pedestal and close the upper cover.

7. Touch the button " BSNA-206 " to start the measurement.

8. Check the results on the screen. The resulting data will be saved automatically and can be searched in the search panel.

Overlay with Nucleic Acids Measurement Results

Up to 5 results can be compared in Overlay interface (Fig. 3-5). Overlay interface is accessible by touching the button " BSNA-206 " in Nucleic Acids measurement interface. Follow procedure of Nucleic Acids measurement to proceed. Each measurement has different colors labeled for graph comparison. Each result will be saved individually, and multiple results can be loaded in Search for comparison. User can return to the measurement interface by touching the button " BSNA-206 ".

Table 8, Procedure for Nucleic Acids Measurement, 1. Enter Nucleic Acids measurement interface by touching the button " " in

Figure 14

3.5 Protein Measurement

In the main operation interface, user can touch the button " BSNA-206 " to open Protein menu . It allows user to choose and access to various measurement options: Bradford, Lowry, BCA, Biuret, Others, and Direct UV.

Table 8, Procedure for Nucleic Acids Measurement, 1. Enter Nucleic Acids measurement interface by touching the button " " in

Figure 15

To close the menu, just retouch the button " BSNA-206 " , or directly touch the button " Table 8, Procedure for Nucleic Acids Measurement, 1. Enter Nucleic Acids measurement interface by touching the button " " in ".

BSNA-206

To enter Bradford measurement interface. (pg. 143)

BSNA-206

To enter Lowry measurement interface. (pg. 14)

BSNA-206

To enter BCA measurement interface. (pg. 14)

BSNA-206

To enter Biuret measurement interface. (pg. 14)

BSNA-206

To enter Others measurement interface, where user can set up the wavelength. (pg. 14)

BSNA-206

To enter Direct UV measurement interface. (pg. 15)

Table 9

3.5.1 Standard Curve

There is Standard Curve interface in Bradford, Lowry, BCA, Biuret, and Others under Protein measurement. It is necessary for the Standard Curve to be set before performing the measurements. User can touch the button " Table 9, 3.5.1 Standard Curve, There is Standard Curve interface in Bradford, Lowry, BCA, Biuret, and Others under Protein " to enter the standard curve measurement interface (Fig. 3-7). Up to 5 concentration points of standard samples for measurements to generate the standard curve. However, the standard curve will be shown after completing measurement of 3 standard samples. User can also save, open, and reset the standard curve data by touching the buttons " Table 9, 3.5.1 Standard Curve, There is Standard Curve interface in Bradford, Lowry, BCA, Biuret, and Others under Protein ", " Table 9, 3.5.1 Standard Curve, There is Standard Curve interface in Bradford, Lowry, BCA, Biuret, and Others under Protein ", and " Table 9, 3.5.1 Standard Curve, There is Standard Curve interface in Bradford, Lowry, BCA, Biuret, and Others under Protein ".

Table 9, 3.5.1 Standard Curve, There is Standard Curve interface in Bradford, Lowry, BCA, Biuret, and Others under Protein

Figure 16

BSNA-206

To enter the measurement interface. It is a default interface when user chose any protein measurement option.

Table 9, 3.5.1 Standard Curve, There is Standard Curve interface in Bradford, Lowry, BCA, Biuret, and Others under Protein

To enter the standard curve interface for a standard curve setting. User can reset, load a standard curve for the measurement, or save current standard curve data.

Conc. (mg/ml)

The concentrations (mg/ml) of Sample 1 to Sample 5.

BSNA-206

To achieve the measurement Abs. values of Sample 1 to Sample 5.

Abs.

Shows the Abs. values of Sample 1 to Sample 5.

Table 9, 3.5.1 Standard Curve, There is Standard Curve interface in Bradford, Lowry, BCA, Biuret, and Others under Protein

Reset all the values in the standard curve interface.

Wavelength (nm)

Shows the wavelength of the current measurement.

Table 9, 3.5.1 Standard Curve, There is Standard Curve interface in Bradford, Lowry, BCA, Biuret, and Others under Protein

To open the saved standard curve data.

Table 9, 3.5.1 Standard Curve, There is Standard Curve interface in Bradford, Lowry, BCA, Biuret, and Others under Protein

To save the current standard curve data and its settings.

Table 10

Procedure for Standard Curve Measurement

1. Prepare at least 3 points of standard samples with different concentration to establish the standard curve.

2. Calibrate the blank.

3. Insert the target standard sample in a cuvette. (Minimum Volume: 1ml)

4. Touch the button " BSNA-206 " to measure.

5. When the measurement is complete, remove the sample.

6. Repeat the steps 3-5, and measure at least 3 standard samples.

3.5.2 Bradford, Lowry, BCA, Biuret, and Others

Besides Direct UV, there are various options for protein sample measurement Bradford, Lowry, BCA, and Biuret with certain measurement wavelength, and Others with a wavelength option. They have almost the same operation steps. In those measurement interface, user should establish a standard curve before the sample measurement.

Table 10, Procedure for Standard Curve Measurement, 1. Prepare at least 3 points of standard samples with different

Figure 17

Table 10, Procedure for Standard Curve Measurement, 1. Prepare at least 3 points of standard samples with different

Figure 18

Table 10, Procedure for Standard Curve Measurement, 1. Prepare at least 3 points of standard samples with different

Figure 19

Table 10, Procedure for Standard Curve Measurement, 1. Prepare at least 3 points of standard samples with different

Figure 20


Figure 21 BSNA-206

Figure 21

Title

Title of the measurement.

Conc.

Shows the concentration of the sample.

Abs.

Shows the Abs. value of the sample.

Wavelength

Shows the wavelength of the current measurement. (In Others protein measurement interface, user can set the measurement wavelength)

Table 11

Procedure for Protein Samples Measurement

1. Enter certain protein measurement interface through the protein menu.

2. Input the title of the measurement.

3. Calibrate the blank.

(In Others protein measurement interface, user should set the wavelength before calibrating the blank)

4. Establish the standard curve.

5. Insert the target sample in a cuvette. (Minimum Volume: 1ml)

6. Touch the button " BSNA-206 " to start the measurement.

7. Check the results on the screen. The resulting data will be saved automatically and can be searched in the search panel.

3.5.3 Direct UV

Direct UV measurement is based on the UV absorption properties of proteins. Unlike other protein sample measurements, the standard curve is not necessary for Direct UV measurement.

Table 11, Procedure for Protein Samples Measurement, 1. Enter certain protein measurement interface through the protein

Figure 22

Title

Title of the measurement.

Conc.

Shows the concentration of the sample.

260nm (10mm path)

Shows the Abs. value at 260nm.

280nm (10mm path)

Shows the Abs. value at 280nm.

Table 12

Procedure for Direct UV Measurement

1. Enter the Direct UV measurement interface (Fig. 3-13).

2. Input the title of the measurement.

3. Calibrate the blank. (Refer to pg. 7)

4. Pipette microvolume of the target sample onto the lower pedestal and close the upper cover.

5. Touch the button " BSNA-206 " to start the measurement.

6. Check the results on the screen. The resulting data will be saved automatically and can be searched in the search panel.

3.6 End Point and OD600 Measurement

End Point and OD600 measurement is to perform spectrometry measurements on samples such as cell culture samples.

Table 12, Procedure for Direct UV Measurement, 1. Enter the Direct UV measurement interface (Fig. 3-13)., 2. Input the title

Figure 24

Table 12, Procedure for Direct UV Measurement, 1. Enter the Direct UV measurement interface (Fig. 3-13)., 2. Input the title

Figure 25

Title

Title of the measurement.

Wavelength

Shows the wavelength of the current measurement. (In End Point measurement interface, user can set the measurement wavelength)

Time

Shows the list of measurement times.

Mode

Shows the list of detection mode of the measurements.

Title (in the data sheet)

Shows the list of measurement titles.

Abs.

Shows the list of measurement Abs. values.

Table 13

Procedure for END Point and OD600 Measurement

1. Enter END Point or OD600 measurement interface (Fig. 3-14 or Fig. 3-15).

2. Input the title of the measurement.

3. Calibrate the blank. (Refer to pg. 8)

(In End Point interface, user should set the wavelength before calibrating the blank)

4. Insert the target sample in a cuvette. (Minimum Volume: 1ml)

5. Touch the button " BSNA-206 " to start the measurement.

6. Check the results on the screen. The resulting data will be saved automatically and can be searched in the search panel.

3.7 Kinetic Measurement

Kinetic measurement is to perform multiple spectrometry measurements on samples in accordance with certain wavelength and time lengths.

Table 13, Procedure for END Point and OD600 Measurement, 1. Enter END Point or OD600 measurement interface (Fig. 3-14 or

Figure 26

Title

Title of the measurement.

Wavelength (nm)

Wavelength of the measurement between 200 -1100 nm.

Measurement Time (min)

Total measurement time.

Interval Time (sec)

Interval time between detections.

Table 14

Procedure for Kinetic Measurement

1. Enter Kinetic measurement interface

2. Input the title of the measurement.

3. Set the wavelength, measurement time and interval time. User may calculate the detection amount by dividing measurement time with interval time and take integer part as the result. (e.g. if the measurement time is set to 1 minute, and interval time is set to 9 seconds, then there will be total 6 detections.)

4. Calibrate the blank. (Refer to pg. 8)

5. Insert the target sample in a cuvette. (Minimum Volume: 1ml)

6. Touch the button " BSNA-206 " to start the measurement.

7. Check the results on the screen. The resulting data will be saved automatically and can be searched in the search panel.

3.8 Spectrum Measurement

Spectrum measurement is to perform spectrometry measurements with specified wavelength range.

Table 14, Procedure for Kinetic Measurement, 1. Enter Kinetic measurement interface, 2. Input the title of the measurement.,

Figure 27

Title

Title of the measurement.

Wavelength (nm)

Input the wavelength range.

BSNA-206

Search Abs value under the specified wavelength.

Table 15

Procedure for Spectrum Measurement

1. Enter Spectrum measurement interface

2. Input the title of the measurement.

3. Set the wavelength range.

4. Calibrate the blank. (Refer to pg. 8)

5. Insert the target sample in a cuvette. (Minimum Volume: 1ml)

6. Touch the button " BSNA-206 " to start the measurement.

7. Check the results on the screen. The resulting data will be saved automatically and can be searched in the search panel.

3.9 Search

User can access to Search panel from any measurement interface or the main interface. All the saved data can be searched in Search panel. There are various options for search. User can select the dates or choose the keywords of the title for the search.

Table 15, Procedure for Spectrum Measurement, 1. Enter Spectrum measurement interface, 2. Input the title of the

Figure 28

Title Search

To find data under the title by matching keywords.

Date & Time

To find data under specified dates and times. User can load a calendar by touching buttons "" and "" and select the date.

Detection Mode

To find data under the selected detection mode.

BSNA-206

Go back to previous page.

BSNA-206

To find all the data under the selected detection mode.

BSNA-206

To find all the data having properties of specified dates, title, and detection mode.

Table 16

Search list panel is accessible by touching the button " BSNA-206 " or " BSNA-206 " in Search panel. In this page, user can check the detailed information of data, edit their titles, delete the selected data, and transfer the data to a USB Flash Drive in FAT format. User also can select data to delete, or transfer, or check the overlay comparison between data. Selected data are checked. User can select to check certain or all the data by touching the button " Table 16, Search list panel is accessible by touching the button " " or " " in Search panel. In this page, user can check " on the left of certain row or the top row in the page. Furthermore, user can go back to the previous search panel and come back to deselect all the data in the entire list of searched data.

Table 16, Search list panel is accessible by touching the button " " or " " in Search panel. In this page, user can check

Figure 29

BSNA-206

To load detailed information of the selected data.

BSNA-206

Open a Change Title panel to change the title of the selected data.

BSNA-206

Delete the selected data.

BSNA-206

Transfer the displayed list to USB Flash Drive. The USB Flash Drive must be in FAT format.

BSNA-206

Go back to previous page.

BSNA-206 / BSNA-206

Select or deselect all the data on the page.

Table 17


3.9.1 Overlay in Search

Up to 5 results can be compared in Overlay interface. It is accessible by selecting multiple data from Nucleic Acids and Spectrum search list panel and touching the button " BSNA-206 ". Each measurement has different color labeled for graph comparison . In Spectrum Overlay panel, the data must share the same wavelength.

3.9.1 Overlay in Search, Up to 5 results can be compared in Overlay interface. It is accessible by selecting multiple data

Figure 30

3.9.1 Overlay in Search, Up to 5 results can be compared in Overlay interface. It is accessible by selecting multiple data

Figure 31

3.9.1 Overlay in Search, Up to 5 results can be compared in Overlay interface. It is accessible by selecting multiple data Go back to previous page.


3.9.2 Data Transfer

Selected data or entire data on the list can be saved as .csv (Microsoft Excel) file in USB Flash Drive by touching " BSNA-206 " in the search list panel. During the process, a USB Flash Drive must be plugged in one of the USB ports on the right side of the instrument. The transferred files will be saved in the Biolab folder. for detailed location of the files. Nucleic Acids, Kinetic, Spectrum, and Direct UV will include Raw Data for each measurement in Raw Data folders.

Procedure for Data Transfer

1. Plug a USB Flash Drive in one of the USB ports on the right side of the instrument.

2. Load files for transferred in Search panel.

3. Select the data to be transferred on the search list. (Skip this step if you are transferring the entire data on the search list)

4. Touch the button " BSNA-206 " to open the transfer option panel

5. Touch " 3.9.2 Data Transfer, Selected data or entire data on the list can be saved as .csv (Microsoft Excel) file in USB Flash Drive " to transfer all the data on the list or " 3.9.2 Data Transfer, Selected data or entire data on the list can be saved as .csv (Microsoft Excel) file in USB Flash Drive " to transfer the selected data.

6. Input a name for the transferred file

7. Find the files in Biolab folder . Each file will be titled as the name input during the process

3.9.2 Data Transfer, Selected data or entire data on the list can be saved as .csv (Microsoft Excel) file in USB Flash Drive 3.9.2 Data Transfer, Selected data or entire data on the list can be saved as .csv (Microsoft Excel) file in USB Flash Drive

Figure 32

3.9.2 Data Transfer, Selected data or entire data on the list can be saved as .csv (Microsoft Excel) file in USB Flash Drive

Figure 33

3.9.2 Data Transfer, Selected data or entire data on the list can be saved as .csv (Microsoft Excel) file in USB Flash Drive

Figure 34

3.10 Account Setting

User can access to Account panel by touching the button "" in the main interface. In Account panel, user can swap the current account, create a new account, edit the selected account, or delete the selected account . Master account cannot be deleted. However, its password can be changed in Setup panel.

3.9.2 Data Transfer, Selected data or entire data on the list can be saved as .csv (Microsoft Excel) file in USB Flash Drive

Figure 35

Current ID

Shows ID of current login account.

BSNA-206

Swap between the current account to the selected account.

BSNA-206

Create a new account.

BSNA-206

Edit ID and passwords of selected account.

BSNA-206

Delete the selected account.

BSNA-206

Return to the main interface.

Table 18

3.11 Power Panel

User can access to Power panel by touching the button " BSNA-206 " in the main interface.

User can put the instrument in a sleep mode. User can also turn off the system in Power panel.

NOTE: IT IS VERY IMPORTANT to turn off the power by touching the button " Table 18, User can access to Power panel by touching the button " " in the main interface., User can put the instrument in a " in Power panel after using the instrument. When it is completely shut down, turn off the power switch at backside of the instrument. If the power is forcibly turned off by the power switch without software shutdown, the recovery mode from the OS might occur during the reboot. User can select the screen design for the sleep mode.

Table 18, User can access to Power panel by touching the button " " in the main interface., User can put the instrument in a

Figure 36

BSNA-206

To put the system in a sleep mode.

Table 18, User can access to Power panel by touching the button " " in the main interface., User can put the instrument in a

Turn off the system.

BSNA-206

Return to the main interface.

Table 19

3.12 Setup

User can access to Setup panel by touching the button " BSNA-206 " in the main interface. User can set the time, update the interface, change Master Password, check System Information, and calibrate the instrument.


NOTE: Setup is not recommended for users to access the panel and change features without manufacturer's supervision.


Figure 37 BSNA-206

Figure 37

BSNA-206

Change the time in the system.

BSNA-206

Update the interface.

BSNA-206

Delete all the data in the system, however, the accounts will remain.

Calibration

Adjust the setting of the system.

DO NOT CHANGE THE SETTINGS without manufacturer's supervision.

BSNA-206

Change the master password.






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