Double Beam UV Visible Spectrophotometer BHV1E3

Spectrophotometer, Double Beam Spectrophotometer
  • Sea, Air, Door to Door Shipping
  • 1 Year Warranty
  • US & European Standards



Double Beam UV Visible Spectrophotometer 190-1100 nm 0.5/1/2/4/5 nm

Specifications

ModelBHV1E3
Wavelength Range190-1100 nm
Spectral Bandwidth0.5/1/2/4/5 nm
Optical SystemDouble Beam, Grating 1200 lines/mm
Wavelength Accuracy±0.3 nm
Wavelength Repeatability0.2 nm
Scan SpeedHi, MED., LOW., MAX. 3000 nm/min
Photometric Accuracy±0.3 %T or ±0.003A @1A
Photometric Range0-200%T,-0.3-3A
Stray Light0.04%T @220 nm, 360 nm
Stability0.0003 A/h @500 nm
Display5 inches LCD (320x240 dots)
Baseline Flatness±0.0005 A
Standard Cell HolderStandard 10 mm single cell holder (2 pcs)
Sample CompartmentStandard 10 mm pathlength cuvette
Light SourceTungsten & Deuterium lamp (Pre-aligned)
USB Type A PortUSB Type A for USB memory device (Right side)
USB Type B PortUSB Type B for optional computer connectivity (Back)
Output Parallel PortParallel port for printer
Power RequirementAC 110/220 V 50/60 Hz
Dimensions (LxWxH)589x428x200 mm
Weight22 kg

Description

It is an advanced double beam design consisting of 3 models.
The two detectors measure sample and reference respectively and simultaneously for optimizing measurement accuracy.
They provide excellent performance for measurements in the range of 190 to 1100 nm, the memory is 32K. They are suitable for pharmaceutical, biochemical and clinical lab applications as well as routine applications such as quantitative analysis, kinetics, wavelength scan, multiple components and DNA/Protein. PC Windows application software makes these instruments versatile. All instruments provide excellent performance for measurements.

Features

Fixed or variable slits (Bandwidths).
For stand-alone models, all software methods are included as built-in standard; this eliminates the need of software.
Online software upgrading via the internet helps to keep it updated.
Data Download-to-PC software expands the data storage to unlimited.
The stand-alone model has a 5-inch screen and the PC model has UV-Vis Analyst software.
Data can be saved by a USB memory device directly.
Stand-alone models of UV-6 Series have the same functions as UV-3 series; see next page for details.


Brief Introduction:
All methods are included as built-in standard; this eliminates the need for software. Online software update via the internet.
The local control software includes functions such as: Photometry, Quantitative, Wavelength Scan, Kinetics, DNA/Protein, Multi-wavelength Test and System Utilities.
Fixed or variable slits (Bandwidths). For stand-alone models, all software methods are included as built-in standard; this

Standard Curve:
Up to 10 standard solutions may be used to establish a calibration equation curve. There is a choice of four methods for fitting curves through the calibration points: Linear fit, Linear fit through zero, square fit and cubic fit.
Standard Curve: Up to 10 standard solutions may be used to establish a calibration equation curve. There is a choice of four

Multi-Wavelength:
Up to 10 wavelengths may be entered, allowing the measurement of multiple wavelengths on a series of samples.
Multi-Wavelength: Up to 10 wavelengths may be entered, allowing the measurement of multiple wavelengths on a series of

Wavelength Scan:
The Wavelength Scan intervals are 0.1, 0.2, 0.5, 1, 2, 5 nm, and High, Medium and Low scan speeds are available. Scan speeds vary from 100 to 3000 nm/min. Wavelengths are scanned from high to low so that the instrument stands-by at high wavelength. This minimizes the degradation of UV sensitive samples. Precise control of filter and lamp changes means that their effects are not seen on the final scan. Post-run manipulation includes re-scaling axes, curve tracking and peak picking.
Wavelength Scan: The Wavelength Scan intervals are 0.1, 0.2, 0.5, 1, 2, 5 nm, and High, Medium and Low scan speeds are

Kinetics:
This mode may be used for scanning time courses or reacting rate calculations. Abs. VS Time graphs are displayed on the screen in real time.
Wait time and measurement time up to 12 hours may be entered with time intervals of 0.5, 1, 2, 5, 10, 30 seconds and 1 min.
Post-run manipulation includes re-scaling, curve tracking and selection of the part of the curve required for the rate calculation. Rate is calculated using a linear regression algorithm before multiplying by the entered factor.
Kinetics: This mode may be used for scanning time courses or reacting rate calculations. Abs. VS Time graphs are displayed

DNA/Protein Test:
Concentration and DNA purity are calculated by Absorbance ratios 260 nm/280 nm or 260 nm/230 nm with optional subtracted absorbance at 320 nm.
DNA Concentration = 62.9A260 - 36.0A280 or 49.1A260 - 3.48A230
Protein Concentration = 1552A260 - 757.3A280 or 183A220 - 75.8A230
Other wavelengths and factors may be entered.
DNA/Protein Test: Concentration and DNA purity are calculated by Absorbance ratios 260 nm/280 nm or 260 nm/230 nm with


UV Analyst PC-Control Software:

The PC application software offers:
Photometric Mode
Quantitative test (Standard curve)
Wavelength Scan
Kinetics
DNA/Protein
Multi-Wavelength
System Utility
The PC application software UV-Vis Analyst takes the best features of the stand-alone version plus more powerful data processing, expanded data collecting, and storage capability. It comes standard with UV3/6 series PC models and is optional to stand-alone models.

Quantitative Test (Standard curve):
Use up to 20 standards to establish a standard curve. Four methods for fitting a curve:
Linear fit.
Linear through zero.
Square fit.
Cubic fit.
Quantitative Test (Standard curve): Use up to 20 standards to establish a standard curve. Four methods for fitting a curve:

Multi-wavelength:
Up to 20 wavelengths can be selected and multiple samples can be measured. (Auto cell changer is required to run multiple samples automatically)
Multi-wavelength: Up to 20 wavelengths can be selected and multiple samples can be measured. (Auto cell changer is required

Wavelength Scan:
Automatically record peaks and valleys. The quantity of channels is unlimited; you can simultaneously store as many as desired.
Post-run manipulation and processing includes:
Re-scaling axes, curve.
1 to 4 derivative.
Smoothing, combination, zooming, overlap.
Wavelength Scan: Automatically record peaks and valleys. The quantity of channels is unlimited; you can simultaneously store

Kinetics (Abs. VS Time):
The Kinetics mode may be used for scanning time courses or reacting rate calculations. Abs. VS Time graphs are displayed on the screen in real time. Wait time, measurement time and time intervals may be entered.
Post-run manipulation includes re-scaling, curve tracking and selection of the part of the curve required for the rate calculation. Rate is calculated using a linear regression algorithm before multiplying by the entered factor.
Kinetics (Abs. VS Time): The Kinetics mode may be used for scanning time courses or reacting rate calculations. Abs. VS Time

DNA/Protein:
Concentration and DNA purity are quickly and easily calculated:
Absorbance ratios 260 nm/280 nm with optional subtracted absorbance at 320 nm.
DNA Concentration = 62.9A260 - 36.0A280
Protein Concentration = 1552A260 - 757.3A280
Other wavelengths and factors may be entered.
DNA/Protein: Concentration and DNA purity are quickly and easily calculated: Absorbance ratios 260 nm/280 nm with optional

Accessories For Purchase

imagesnameog_model
imagesnameog_modelog_pricepriceMicro Cell Holder(Beam height: 15mm)9002408-Position Auto Cell Changer9003104-Cell Holder for
Micro Cell Holder
(Beam height: 15mm)
900240
imagesnameog_modelog_pricepriceMicro Cell Holder(Beam height: 15mm)9002408-Position Auto Cell Changer9003104-Cell Holder for
8-Position Auto Cell Changer
900310
imagesnameog_modelog_pricepriceMicro Cell Holder(Beam height: 15mm)9002408-Position Auto Cell Changer9003104-Cell Holder for
4-Cell Holder for 10mm SQU.cuvette
900410
imagesnameog_modelog_pricepriceMicro Cell Holder(Beam height: 15mm)9002408-Position Auto Cell Changer9003104-Cell Holder for
4-Cell Holder for 50mm SQU.cuvette
900420
imagesnameog_modelog_pricepriceMicro Cell Holder(Beam height: 15mm)9002408-Position Auto Cell Changer9003104-Cell Holder for
4-Cell Holder for 100mm SQU.cuvette
900430
imagesnameog_modelog_pricepriceMicro Cell Holder(Beam height: 15mm)9002408-Position Auto Cell Changer9003104-Cell Holder for
Square Cuvettes
Glass :10 mm
916101
QUARTZ: 10 mm 916111
20 mm 916112 30 mm 916113
50 mm 916114 100 mm 916115
20 mm
916102
30 mm
916103
50 mm
916104
100 mm
916105
Square cuvettes Quartz:10 mm
916111
20 mm
916112
30 mm
916113
50 mm
916114
100 mm
916115
imagesnameog_modelog_pricepriceMicro Cell Holder(Beam height: 15mm)9002408-Position Auto Cell Changer9003104-Cell Holder for
Micro cell, Quartz
(Beam height: 15mm)
100UL
916126
200UL
916127
500UL
916123
imagesnameog_modelog_pricepriceMicro Cell Holder(Beam height: 15mm)9002408-Position Auto Cell Changer9003104-Cell Holder for
Sipper System
900140
imagesnameog_modelog_pricepriceMicro Cell Holder(Beam height: 15mm)9002408-Position Auto Cell Changer9003104-Cell Holder for
Constant-Temperature System
900150
imagesnameog_modelog_pricepriceMicro Cell Holder(Beam height: 15mm)9002408-Position Auto Cell Changer9003104-Cell Holder for
Constant-Temperature Sipper System
900160
imagesnameog_modelog_pricepriceMicro Cell Holder(Beam height: 15mm)9002408-Position Auto Cell Changer9003104-Cell Holder for
Test Tube Holder
900530
imagesnameog_modelog_pricepriceMicro Cell Holder(Beam height: 15mm)9002408-Position Auto Cell Changer9003104-Cell Holder for
Cylindrical Cell Holder
900540
imagesnameog_modelog_pricepriceMicro Cell Holder(Beam height: 15mm)9002408-Position Auto Cell Changer9003104-Cell Holder for
Solid Sample Holder (Single Cell)
900550
imagesnameog_modelog_pricepriceMicro Cell Holder(Beam height: 15mm)9002408-Position Auto Cell Changer9003104-Cell Holder for
Water-Jacketed Cell Holder
900610
imagesnameog_modelog_pricepriceMicro Cell Holder(Beam height: 15mm)9002408-Position Auto Cell Changer9003104-Cell Holder for
10mm Water-Jacketed
4-Cell Holder
900620
imagesnameog_modelog_pricepriceMicro Cell Holder(Beam height: 15mm)9002408-Position Auto Cell Changer9003104-Cell Holder for
Milas Deuterium
Lamp
916633
imagesnameog_modelog_pricepriceMicro Cell Holder(Beam height: 15mm)9002408-Position Auto Cell Changer9003104-Cell Holder for
Halogen Lamp(Philips)
6V10W: 911634
imagesnameog_modelog_pricepriceMicro Cell Holder(Beam height: 15mm)9002408-Position Auto Cell Changer9003104-Cell Holder for
Halogen Lamp(Philips)
12V20W: 916634
imagesnameog_modelog_pricepriceMicro Cell Holder(Beam height: 15mm)9002408-Position Auto Cell Changer9003104-Cell Holder for
Halogen Lamp(Osram)
961634
imagesnameog_modelog_pricepriceMicro Cell Holder(Beam height: 15mm)9002408-Position Auto Cell Changer9003104-Cell Holder for
Self Masking Cont. Flowthrough G.Cell
(Beam height: 15mm)
5mm
916135
10mm
916136
20mm
916137
30mm
916138
Self Masking Cont. Flowthrough Q. Cell
(Beam height: 15mm)
5mm
916145
10mm
916146
20mm
916147
30mm
916148
imagesnameog_modelog_pricepriceMicro Cell Holder(Beam height: 15mm)9002408-Position Auto Cell Changer9003104-Cell Holder for
Thermal Printer
920910





Request Quote
Name*:
Email*:
I am *: Distributor End User Others
WhatsApp :
Product :
Subject :
Message :